| 精品肝素钠的制备及效价分析 |
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| 引用本文： | 李红心. 精品肝素钠的制备及效价分析[J]. 陕西科技大学学报, 2005, 23(5): 32-34,40 |
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| 作者姓名： | 李红心 |
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| 作者单位： | 陕西科技大学生命科学与工程学院,陕西咸阳712081 |
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| 摘 要： | 采用过氧化氢二次氧化法精制肝素钠，在此过程中采用低温离心技术解决了去除杂蛋白时沉淀不易滤除的困难；通过实验确立了酶解结合氧化法精制肝素钠的新工艺，克服了粗品肝素钠纯化过程中残留杂蛋白难以除去的缺点；在粗品肝素钠溶液中加入胰蛋白酶对杂蛋白进行酶解，再结合氧化除杂，提高了精品肝素钠的效价和效价回收率。
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| 关 键 词： | 肝素钠 精制 过氧化氢 胰蛋白酶 |
| 文章编号： | 1000-5811（2005）05-0032-03 |
| 收稿时间： | 2005-03-15 |
| 修稿时间： | 2005-03-15 |
PURE HEPARIN SODIUM PREPARATION AND ACTIVITY DETERMINING |
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| LI Hong-xin. PURE HEPARIN SODIUM PREPARATION AND ACTIVITY DETERMINING[J]. Journal of Shaanxi University of Science & Technology(Natural Science Edition), 2005, 23(5): 32-34,40 |
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| Authors: | LI Hong-xin |
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| Abstract: | The purification of heparin by double oxidation process with H2O2 was investigated. The centrifugal technique under low temperature was used to solve the difficulty in removing the precipitation. The crude heparin sodium salt was degraded by adding trypsin, so that the residual proteins were removed from heparin. The results showed that the improved process,which combined oxidation and degradation,can raise the potency and recovery of heparin sodium salt. |
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| Keywords: | heparin sodium purification hydrogen peroxide trypsin |
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