| 基于生物发光的高灵敏度ATP自动测试系统研究 |
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| 引用本文： | 岳伟伟,何保山,周爱玉,罗金平,蔡新霞.基于生物发光的高灵敏度ATP自动测试系统研究[J].高技术通讯,2009,19(6). |
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| 作者姓名： | 岳伟伟 何保山 周爱玉 罗金平 蔡新霞 |
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| 作者单位： | 1. 中国科学院电子学研究所传感技术国家联合重点实验室,北京,100080
2. 中国科学院研究生院,北京,100080 |
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| 基金项目： | 863计划，国家自然科学基金，北京市科委 |
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| 摘 要： | 针对高灵敏度、宽响应范围及自动检测三磷酸腺苷(ATP)的需求,设计了一种基于生物发光原理的ATP检测系统.系统采用自动加样技术,在发光反应的同时开始记录光强,使仪表检测自动化,且提高检测灵敏度;在优化光电检测单元设计和减小杂散光及电磁干扰的同时,提高荧光检测效率;通过电路和数据处理算法优化提高仪表检测范围.以浓度为10-15～10-6 M范围内的标准ATP溶液进行了实验,结果表明仪表测量光强与ATP实际浓度相关系数为0.974,检测范围达9个数量级.与市场相关仪器相比,该系统具有自动化测试,性能和灵敏度高、响应范围宽的特点,在ATP测量以及相关领域如细菌测量、毒性测试及卫生状况检测等方面具有广泛的应用前景.
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| 关 键 词： | 三磷酸腺苷(ATP) 生物发光 检测系统 自动加样 |
Study of an automatic ATP detecting system with high sensitivity based on bioluminescence |
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| Yue Weiwei,He Baoshan,Zhou Aiyu,Luo Jinping,Cai Xinxia.Study of an automatic ATP detecting system with high sensitivity based on bioluminescence[J].High Technology Letters,2009,19(6). |
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| Authors: | Yue Weiwei He Baoshan Zhou Aiyu Luo Jinping Cai Xinxia |
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| Affiliation: | Yue Weiwei He Baoshan Zhou Aiyu Luo Jinping Cai Xinxia(State Key Laboratory of Transducer Technology,Institute of Electronics,Chinese Academy of Sciences,Beijing 100080)(Graduate University of Chinese Academy of Sciences,Beijing 100080) |
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| Abstract: | To meet the needs of automatic adenosine triphosphate(ATP)detection with high sensitivity and wide response range,an ATP detection system was designed based on bioluminescence.By recording the light intensity at the beginning of the bioluminescence reaction using the automatic injection technology,the system can detect samples automatically with high sensitivity.The photo-electronic detection unit was optimized to decrease the influence of stray light and electromag- netism and increase the efficiency of lu... |
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| Keywords: | adenosine triphosphate (ATP) bioluminescence detection system automatic injection |
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