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反相高效液相色谱法测定蜂王浆中的10-羟基-2-癸烯酸
引用本文:杨彬,郝莉花,陈欣欣. 反相高效液相色谱法测定蜂王浆中的10-羟基-2-癸烯酸[J]. 现代食品科技, 2007, 23(7): 92-93
作者姓名:杨彬  郝莉花  陈欣欣
作者单位:河南省产品质量监督检验院,河南,郑州,450004;河南省产品质量监督检验院,河南,郑州,450004;河南省产品质量监督检验院,河南,郑州,450004
摘    要:采用反相高效液相色谱法测定蜂王浆中的10-羟基-2-癸烯酸.采用ZORBAX Eclipse XDB-C18柱,以甲醇:磷酸1%溶液(50:50, v/v)为流动相,流速为1.0 mL/min,检测波长210 nm.该法简便、快速、重现性好、回收率高.

关 键 词:高效液相色谱法  10-羟基-2-癸烯酸  蜂王浆
文章编号:1673-9078(2007)07-0092-02
收稿时间:2007-04-02
修稿时间:2007-04-02

Determination of 10-Hydroxy-2-Decenoic Acid in Royal Jelly by Reverse High Performance Liquid Chromatography
YANG Bin,HAO Li-hu,CHEN Xin-xin. Determination of 10-Hydroxy-2-Decenoic Acid in Royal Jelly by Reverse High Performance Liquid Chromatography[J]. Modern Food Science & Technology, 2007, 23(7): 92-93
Authors:YANG Bin  HAO Li-hu  CHEN Xin-xin
Affiliation:Henan Institute of Product Quality Supervision and Inspection, Zhengzhou 450004, China
Abstract:Reverse high performance liquid chromatography was used for the determination of 10-hydroxy-2-decenoic acid in royal jelly.Chromatographic separation was performed using ZORBAX Eclipse XDB-C18 column with methanol and 1% phosphoric acid(50:50,V/V) as mobile phase at a flow rate of 1.0 mL/min.The UV detection wavelength was fixed at 210 nm.This method was proved to be simple,rapid and has good reproduction quality and high recovery rate.
Keywords:high performance liquid chromatography   10-hydroxy-2-decenoic acid   royal jelly
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