3D level set method for blastomere segmentation of preimplantation embryos in fluorescence microscopy images |
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Authors: | Andrey Grushnikov Ritsuya Niwayama Takeo Kanade Yasushi Yagi |
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Affiliation: | 1.Yagi Laboratory, Department of Intelligent Media, The Institute of Scientific and Industrial Research,Osaka University,Ibaraki,Japan |
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Abstract: | Detailed and accurate characteristics of preimplantation embryos are fundamental for a deep understanding of their development. Recent studies indicate that various geometric features of cells, such as size, shape, volume, and position play a significant role in embryo growth. However, a quantitative assessment of these characteristics first needs a segmentation of the individual cells. The manual separation and labeling of cells is extremely inefficient, and an automated approach is highly desirable. This paper presents an automatic method for early stage embryo segmentation into its constituent cells and membranes using three-dimensional (3D) data. The input data consist of two Z-stacks of fluorescence microscope images containing nuclei and membranes. The method uses a 3D level set segmentation algorithm. Its evaluation is based on a dataset composed of 20 mouse embryos, each with 4–32 blastomeres. Segmentation accuracy was evaluated by calculating F-scores with ground truth obtained by manually labeling desired regions. We also compared output of our method with the one acquired with a watershed algorithm. The proposed approach was able to achieve more than (90%) accuracy for embryos with 4 and 8 cells, while for embryos with higher number of cells it was lower, reaching (75%) for 32-cell embryo. |
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