| 离子色谱电化学测定牛奶中的乳糖和乳果糖 |
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| 引用本文： | 曾文芳,时巧翠,陈永欣,朱京平,朱岩. 离子色谱电化学测定牛奶中的乳糖和乳果糖[J]. 食品科学, 2006, 27(5): 205-207 |
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| 作者姓名： | 曾文芳 时巧翠 陈永欣 朱京平 朱岩 |
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| 作者单位： | 浙江大学西溪校区化学系 |
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| 基金项目： | 中国科学院资助项目;浙江省自然科学基金;教育部留学回国人员科研启动基金 |
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| 摘 要： | 本方法成功地对牛奶中的乳糖和乳果糖进行了分离和测定。以CarboPacPA1色谱柱,5mmol/LNaOH+1mmol/LCH3COONa等梯度淋洗,脉冲安培检测,该方法具有良好的重现性、线性关系(r2>0.9989)和较低的检测限。乳糖和乳果糖的检测限分别是:0.012、0.02mg/L;样品测定的回收率为95%～102.8%。
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| 关 键 词： | 脉冲安培检测 乳糖 乳果糖 离子色谱 |
| 文章编号： | 1002-6630（2006）05-0205-03 |
| 收稿时间： | 2005-06-30 |
| 修稿时间： | 2005-06-30 |
Determination of Lactose and Lactulose by Ion Chromatography with Pulsed Amperometric Detection |
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| ZENG Wen-fang,SHI Qiao-cui,CHEN Yong-xin,ZHU Jing-ping,ZHU Yan. Determination of Lactose and Lactulose by Ion Chromatography with Pulsed Amperometric Detection[J]. Food Science, 2006, 27(5): 205-207 |
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| Authors: | ZENG Wen-fang SHI Qiao-cui CHEN Yong-xin ZHU Jing-ping ZHU Yan |
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| Affiliation: | Department of Chemistry, Xixi Campuus of Zhejiang University, Hangzhou 310028, China |
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| Abstract: | In this paper, a new method for simultaneous determination of lactose and lactulose by using ion chromatography. CarboPac PA1 analytical column(2mm×250mm) and CarboPac PA1 guard column(2mm×50mm), and 5mmol/L NaOH+1mmol/L CH3COONa, stepwise elution with pulsed amperometric detection has been described. The very low conductance background could be got with the sensitivity of lactose and lactulose greatly improved. By this method, good linear relationship, sensitivity and reproducibility were obtained(r2>0.9989). The detection limits (S/N=3) of lactose and lactulose are 0.012, 0.02mg/L respectively and the recovery of sample in sample A and B ranges from 95%～102.8%。 |
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| Keywords: | pulsed amperometric detection lactose lactulose ion chromatography |
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