RAPD在灰平菇菌株亲缘关系研究中的应用

采用RAPD技术分析了5个不同来源的灰平菇栽培菌株的DNA多态性。筛选出的8条随机引物共扩增得到107条带,其中多态性片段为69条,多态性比率为64.4%,说明收集到的灰平菇菌株具有一定的遗传多样性,它们之间存在一定的遗传差异。进一步聚类分析结果显示,当相性达到0.470的水平时,5个菌株聚成2组,第1组为1号(PL-72)、2号(PL-2)、3号(烟平2106)、4号(PL-A2);第2组为5号(PL-AD28)。当相似性达到0.570的水平时,第1组又被分为两个亚组,1号和3号菌株聚为一个亚组,而2号和4号菌株聚为一个亚组。本研究为灰平菇优良品种选育和亲缘关系的研究提供分子生物学依据。

Application of RAPD in Genetic Relationship Analysis of Pleurtus ostreatus Strains

Five cultivated strains of Pleurotus ostreatus from different areas of China were assessed for their DNA polymorphism by RAPD technique. A total of 107 RAPD bands were amplified by eight selected primers, in which 69 bands were polymorphic and the rate was 64.4%. The variations of RAPD band patterns and DNA similarity among the strains revealed that there is definite genetic diversity in the natural germplasm of Pleurotus ostreatus in China. A cluster dendrogram of these samples showed that the five strains were classified into 2 groups using similarity of 0.470 as a cut-off point. Groupl consisted of PL-72, PL-2, Yanping 2106 and PL-A2, and group two was PL-AD28. While using 0.570 as a cut-off point, the group 1 was further classified two subgroups. One was comprised of PL-72 and Yanping 2106, and the other included PL-2 and PL-A2. This study provide molecular evidence for selection and breeding of eminent cultivars and genetic relationship in Pleurotus ostreatus.