加氧酶催化生物转化研究最新进展

加氧酶是可以将单个或双个氧原子加入到一个有机化合物分子中的一类酶,且具有选择性生物氧化特性,所以被广泛研究。用蛋白质工程来改造血红素和黄素单加氧酶,可以改变它们底物的特性,或增强加氧酶的稳定性及其对映异构体或区域选择性。在工业上,正尝试着将加氧酶所催化的生物转化技术进行放大应用,但辅因子再生仍是影响这类酶工业化应用的关键问题。通过蛋白质工程还可以进一步地弄清加双氧酶的结构与功能的关系。

Recent Progress in Oxygenase-catalyzed Biotransformations

Oxygenases are enzymes that introduce one or two atoms of molecular oxygen into an organic molecule and can oxidate organic compounds selectively.So oxygenases continue to be widely studied.Protein engineering has resulted in heme and flavin monooxygenases with widely altered substrate specificities,or has increased their stability and improved their regioselectivity or enantioselectivity.In the industry,many attempts have been made to scale-up reactions catalyzed by these enzymes.Cofactor regeneration is still a key issue in these developments.Protein engineering also contributed to understanding of structure versus function in dioxygenases.