序批式活性污泥法反硝化聚磷菌的培养驯化研究

以某污水处理厂活性污泥作为种泥,采用序批式活性污泥法(SBR)进行反硝化聚磷菌(DPB)培养驯化研究。结果表明,经过厌氧-好氧,厌氧-换水-缺氧,厌氧-缺氧,厌氧-缺氧-短时曝气4个阶段培养驯化,系统出水效果良好:出水PO43--P的质量浓度稳定在0.5 mg.L-1以下,平均除磷率达96%;出水COD稳定在50 mg.L-1以下,平均去除率达77%。DPB占聚磷菌的比例约为65.02%。当投加不同含量的NO3--N时,硝酸盐的含量只影响吸磷速率而不影响吸磷量。当缺氧段DPB体内的PHB为限制因素时,缺氧吸磷过程在不同NO3--N含量下基本相同。

ENRICHMENT AND CULTIVATION OF DENITRIFYING PHOSPHORUS REMOVING BACTERIA IN SEQUENCING BATCH REACTOR

Denitrifying phosphorus removing bacteria(DPB) was enriched and cultured using activated sludge from a wastewater treatment plant in a set of sequencing batch reactor(SBR).Results showed that through operating stably under the four-step process of anaerobic/aerobic and anaerobic/water-changing/anoxic and anaerobic/anoxic and anaerobic/anoxic/ short-time aeration,the system had a good effluent performance.The average removal rate of PO43——P and COD was 96% and 77% respectively when the effluent concentration was below 0.5 mg·L-1 and 50 mg·L-1 respectively.The proportion of denitrifying phosphate removing bacteria to phosphorus accumulating organism was about 65.02% through the four-step process.The rate of PO43——P uptake was affected by the different concentration of NO3——N while the amounts of PO43——P uptake did not changed with the variety of the nitrite concentration.The change of anoxic PO43——P uptake process was similar in different nitrite concentration when the PHB was the only limiting factor of the DPB in anoxic stage.