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生物破乳菌的培养及代谢产物分析
引用本文:刘娜,郭继香,王晓梅.生物破乳菌的培养及代谢产物分析[J].应用化工,2007,36(6):557-560,563.
作者姓名:刘娜  郭继香  王晓梅
作者单位:1. 中国石化胜利工程设计咨询有限责任公司,山东,东营,257026
2. 中国石油大学,提高采收率中心,北京,102249
3. 中国科学院,成都生物研究所,四川,成都,610041
基金项目:中国石油化工股份有限公司资助项目
摘    要:从胜利油田采出水及其它样品中筛选得到破乳效果较好的D3破乳菌,考察了该破乳菌的培养条件及代谢产物。结果表明,D3菌的适宜生长条件为:温度40℃,pH 7~9,培养时间72 h。D3菌为革兰氏阴性细菌,归为沙雷氏菌属;其代谢产物主要含有:鼠李糖酯、脂肽类、醛、胺。D3菌质量浓度10 g/L时,油水的界面张力由7.65 mN/m降至0.98 mN/m;当菌浓度在0.5~1.0 g/L时,含油污水的除油率大于50%。

关 键 词:生物破乳菌  代谢产物  培养  分析
文章编号:1671-3206(2007)06-0557-04
修稿时间:2007-04-15

Training of biodemusifer and analysis of metabolic product
LIU Na,GUO Ji-xiang,WANG Xiao-mei.Training of biodemusifer and analysis of metabolic product[J].Applied chemical industry,2007,36(6):557-560,563.
Authors:LIU Na  GUO Ji-xiang  WANG Xiao-mei
Affiliation:1. Shengli Engineering Design and Consulting Company Ltd, SINOPEC, Dongying 257026, China; 2. Enhanced Oil Recovery Research Center, China University of Petroleum, Beijing 102249, China; 3. Chengdu Institute of Biology, Chinese Academy of Science, Chengdu 610041, China
Abstract:D3 biodemusifier was obtained from oil water of Shengli oil field and other oil fields.The better growth condition of D3 biodemusifier is 40 ℃,pH 7~9 and training time 72 h.D3 biodemusifier is Grain-negative bacteria,and it belongs to Serratia.Its metabolite mainly contain rhamnolipid,lipopeptide,aldehyde and amine.When the mass concentration of D3 is 10 g/ L,the interfacial tension is reduced from 7.65 mN/m to 0.98 mN/m.When the mass concentration of D3 is 0.5~1.0 g/L,the rate of removing oil is over 50% from oil wastewater.
Keywords:biodemusifier  metabolite  bring up  analysis
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