人早幼粒白血病细胞裸鼠异种移植模型的傅里叶变换红外光谱探索

应用傅里叶变换红外光谱法对HL-60(人早幼粒白血病细胞)裸鼠异种移植模型癌变及癌旁正常组织粉末样品进行了分析研究.结果表明:(1)甲基CH3和蛋白质分子C-O峰在癌变组织中均有不同程度的频移,(2)正常组织的红外光谱在1746 cm-1处存在较强的吸收峰,而在肿瘤中只有较弱的吸收峰或观察不到吸收峰,(3)癌变组织核酸分子磷酸二脂基团PO2-的对称伸缩振动的相对吸收强度明显增强,(4)正常组织中脂类分子弯曲振动谱带吸收峰相对强度明显强于甲基变角振动谱带的相对强度,而在癌变组织中这两处吸收峰的相对强度大小基本相等.说明傅里叶变换红外光谱法可以用于对裸鼠癌变组织进行鉴别诊断,利用动物异种移植模型进行红外光谱分析可成为肿瘤红外光谱研究的一种新方法.

STUDIES ON FOURIER TRANSFORM IR SPECTRA OF HUMAN MYELOCYTIC LEUKEMIA XENOGRAFTED IN NUDE MICE

Fourier transform infrared spectroscopy(FTIR) was applied to the study of human myelocytic leukemia(HL-60) xenografted in nude mice.Experiment results show that(1) the peak of CH_3 and C-O of molecule of protein shifts to a certain extent in cancerous tissues,(2) the relative intensity of 1746cm~(-1) is very strong in normal tissues,but it is very weak in malignant tissues,(3) the relative intensity of the stretch vibration of PO_2~-in phosphodiester group is obviously strong,(4) the ratio of the relative intensity of deformed vibration of the adipose molecule and the deoxyribose vibration of CH_3 was higher in normal tissues,but it is almost equal in malignant tissues.The result might indicate that the FTIR could be used in the clinical diagnoses of the malignant tissues of human myelocytic leukemia(HL-60) xenografted in nude mice,and it also suggests that the nude mice tumor models could be a new method for the FTIR spectroscopic studies of tumor.