氨基酸对红曲霉突变菌株合成代谢黄色素和橘霉素的影响 |
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引用本文: | 田园,鲁华敏,周波,钟海雁.氨基酸对红曲霉突变菌株合成代谢黄色素和橘霉素的影响[J].中国酿造,2014(12):52-57. |
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作者姓名: | 田园 鲁华敏 周波 钟海雁 |
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作者单位: | 稻谷及副产物深加工国家工程实验室;粮油深加工与品质控制湖南省重点实验室;中南林业科技大学食品科学与工程学院; |
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基金项目: | 国家自然科学基金(31301550);湖南省教育厅科学研究项目(14C1185) |
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摘 要: | 本实验研究了氨基酸对红曲黄色素合成代谢的影响.结果表明,除了酪氨酸(4 g/L)和甘氨酸(1 g/L)明显有利于黄色素合成代谢外,其余所选氨基酸都不利于黄色素的合成代谢或影响不明显,随着各种氨基酸的添加量从1g/L增加到6g/L,黄色素色价减少量都≥20%,有些黄色素减少量甚至达到83.15%(如苯丙氨酸).甘氨酸添加量为6g/L时,橘霉素的合成代谢量是对照组的6.61倍,提高561%,橘霉素生成量为3.37 mg/L,其余氨基酸能明显消除橘霉素的合成代谢或影响不明显.从工业化生产黄色素角度考虑,通过添加4g/L的酪氨酸不仅有利于红曲霉突变菌株合成代谢黄色素,还能降低橘霉素的代谢生成量.
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关 键 词: | 氨基酸 橘霉素 黄色素 红曲霉突变菌株 |
Effect of amino acid on yellow pigments and citrinin production by Monascus anka mutant |
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Affiliation: | TIAN Yuan,LU Huamin,ZHOU Bo,ZHONG Haiyan( 1.National Engineering Laboratory of Rice and By-products Processing, Changsha 410004, China;Hunan Province Key Laboratory of Grain & Oil Processing and Quality Control, Changsha 410004, China;School of Food Science and Engineering, Central South University of Forestry and Technology, Changsha 410004, China) |
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Abstract: | The effect of amino acids on anabolism of Monascus yellow pigments was studied.Results showed that all amino acids,except for tyrosine (4 g/L) and glycine (lg/L),were obviously disadvantageous to yellow pigments anabolism by Monascus anka mutant.With the amino acid addition increasing from lg/L to 6g/L,Monascus yellow pigments color value reduced more than 20%,some even reduced 83.15% (e.g.phenylalanine).When the glycine addition was 6 g/L,the citrinin production was 3.37 mg/L,which was 6.61 times of the control group and increased by 561%.Other amino acids went against citrinin anabolism or have no significant effect.In terms of Monascus yellow pigment industrial production,tyrosine (4 g/L)can not only promote yellow pigments production from Monascus anka mutant,but also eliminate citrinin production. |
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Keywords: | amino acid citrinin yellow pigments Monascus anka mutant |
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