| 木薯原料柠檬酸发酵补料新工艺的研究 |
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| 引用本文： | 洪厚胜,叶宇,张庆文,刘辰,华敏. 木薯原料柠檬酸发酵补料新工艺的研究[J]. 食品与发酵工业, 2005, 31(12): 22-25 |
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| 作者姓名： | 洪厚胜 叶宇 张庆文 刘辰 华敏 |
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| 作者单位： | 1. 南京工业大学制药与生命科学学院,南京,210009
2. 南通华泽生物化学有限公司,南通,226011 |
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| 摘 要： | 选择计量罐式连续流加补料系统,采用新选育的黑曲霉(Aspergillus niger)Wml-016菌株在50 L自动发酵罐上研究间歇补料对发酵的影响。利用中心复合设计和响应面分析方法,实验得出木薯原料柠檬酸补料发酵的优化条件:发酵到第36 h开始进行4次流加补料,间隔时间为1.5 h,补入DE值为80的木薯水解液和质量浓度为0.04%的(NH4)2SO4。采用上述工艺发酵产酸率达到18.5%,残糖为0.2%,周期仅为64 h,结果明显优于分批发酵。
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| 关 键 词： | 木薯 柠檬酸发酵 补料 中心复合设计 响应面 |
| 收稿时间： | 2005-05-18 |
| 修稿时间： | 2005-09-16 |
Study on a New Method of Citric Acid Fed- batch Fermentation Using Cassava as Raw Material |
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| Hong Housheng,Ye Yu,Zhang Qingwen,Liu Chen,Hua Min. Study on a New Method of Citric Acid Fed- batch Fermentation Using Cassava as Raw Material[J]. Food and Fermentation Industries, 2005, 31(12): 22-25 |
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| Authors: | Hong Housheng Ye Yu Zhang Qingwen Liu Chen Hua Min |
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| Affiliation: | 1,College of Pharmacy and Life Science, Nanjing University of Technology, Nanjing 210009 ,China;2,Nantong Huaze Biochemistry Co. Ltd., Nantong 226011 ,China |
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| Abstract: | The effect of fed batch system with the measuring tank on the fermentation of a newly selected strain,Aspergillus niger wml-016,was studied in a 50 L automatic fermentor.Optimal conditions were obtained from the experiments with methods of central composite design and response surface analysis.The feeding is commenced at 36th hour at intervals of 1.5hours for four times,and the DE of the feeding cassava hydrolyte is 80,while the concentration of feeding(NH_4)_2SO_4 is 0.04%(w/v).By the above mentioned techmiques,the citric acid productivity is up to 18.5%;the residue glucose is 0.2%,while the fermentation period is only 64 hours.And the process is obviously much better than traditional fermentation. |
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| Keywords: | cassava citric acid fermentation fed- batch central composite design response surface |
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