香蕉及茶叶中单宁降解菌株的筛选

本实验从香蕉茎叶、茶叶及香蕉土壤、茶土壤环境中经初筛和复筛分离纯化出16株产单宁酸酶的菌株，对其菌株的外在特性和产酶活力进行了研究和测定。结果表明，从培养基来看，产酶菌株以从牛肉膏蛋白胨中性培养基里分离出来的居多（11株）；从原料来源看，以从茎叶分离出的菌株居多（14株）；菌株多为棕或绿色霉菌（15株）；水解圈以透明的居多（10株），且16株菌株的水解圈与菌落直径比均在1．0．2．0之间。产酶菌株的酶活和水解圈的透明状态基本相符；其中以牛中香茎处10^0-1、牛中香茎处10^0-2、牛中香茎处10^0-6等3株的酶活值最大，为3．4。

Isolation of Tannase-producing Bacterias from Leaves of Banana and Tea

In this study,16 powerful tannase-producing bacterias were isolated from leave or soil of banana and tea. We investigated their characteristic and tannase-producing activity .The result are as follow,the 16 powerful tannase-producing bacterias are mostly isolated from the beef and peptone neutral culture,leaves; most of them are brown or green mildew; hydrolyse zones are mostly transparent and the scale of D/d is mostly between 1.0 and 2.0.The tannase-producing activity of these bacterias are accordance with the state of their hydrolyse zones. The tannase-producing activities of Niuzhong-xiangjingchu 100-1,100-2,100-6 are the most,reach 3.4.