Interference of Saturated Fats in the Determination of Low Levels of <Emphasis Type="Italic">trans</Emphasis> Fats (below 0.5%) by Infrared Spectroscopy |
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Authors: | M M Mossoba J K G Kramer V Milosevic M Milosevic H Azizian |
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Affiliation: | (1) Food and Drug Administration, Center for Food Safety and Applied Nutrition, Mail Stop HFS-717, Room BE-012, 5100 Paint Branch Parkway, College Park, MD 20740-3835, USA;(2) Agriculture and Agri-Food Canada, Guelph, ON, Canada;(3) MeV Photonics, Westport, CT, USA;(4) NIR Technologies, Oakville, ON, Canada |
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Abstract: | The mandate to label food products with the content of total trans fatty acids has led to an increase in demand for sensitive and accurate methodologies for the rapid quantitation of trans fats. Unfortunately, the latest official infrared (IR) spectroscopic method lacks the required sensitivity. A more sensitive
IR procedure that requires the measurement of the height of the second derivative (2D) of the trans absorption band at 966 cm−1 was recently proposed; however, a reported inconsistency at low trans levels between GC (0% of total fat) and IR (1.2% of total fat) results for a fully hydrogenated vegetable oil could not be
reconciled, and triggered further investigations. For the first time, we recognize and report the presence of weak interference
bands (962–956 cm−1) attributed to saturated fats in the IR spectra of trans fats; these interference bands have an adverse impact on the sensitivity and accuracy of the IR determination at low trans levels (≤0.5% of total fat). Therefore, weak spectral features observed at energies below the one expected for trans bands (966 cm−1) in test samples high in saturated fat (coconut oil and cocoa butter) must not be mistaken for trans bands. |
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