| 无溶剂体系酶法催化酸解合成共轭亚油酸甘油酯 |
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| 引用本文： | 惠菊,单良,金青哲,刘元法,王兴国. 无溶剂体系酶法催化酸解合成共轭亚油酸甘油酯[J]. 中国油脂, 2009, 34(7) |
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| 作者姓名： | 惠菊 单良 金青哲 刘元法 王兴国 |
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| 作者单位： | 江南大学,食品学院,江苏,无锡,214122 |
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| 摘 要： | 采用商业化固定化酶Novozym 435作为生物催化剂,催化共轭亚油酸(CLA)和葵花籽油的酸解反应合成富含CLA的结构脂质(CLA-SL).研究了在无溶剂体系中,底物摩尔比、酶用量、体系含水量、反应温度和反应时间对产物中CLA含量和Sn-2位CLA含量的影响.结果表明,最佳反应条件为:CLA与葵花籽油摩尔比3 :1,酶用量10%,体系含水量1%,反应温度55 ℃,反应时间36 h.在最佳反应条件下,产物中的CLA含量和Sn-2位CLA含量分别为15.7%和2.73%.
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| 关 键 词： | 固定化酶 酸解 CLA甘油酯 |
Enzymatic synthesis of structured lipid containing conjugated linoleic acid in a solvent-free system |
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| HUI Ju,SHAN Liang,JIN Qingzhe,LIU Yuanfa,WANG Xingguo. Enzymatic synthesis of structured lipid containing conjugated linoleic acid in a solvent-free system[J]. China Oils and Fats, 2009, 34(7) |
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| Authors: | HUI Ju SHAN Liang JIN Qingzhe LIU Yuanfa WANG Xingguo |
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| Abstract: | A structured lipid (SL) containing conjugated linoleic acid (CLA) was prepared using immobilized lipase(Novozym 435)-catalyzed acidolysis of sunflower seed oil with CLA. The acidolysis reaction was carried out in a solvent-free system. The effects on incorporation of CLA, and the CLA content in Sn-2 position of water content, molar ratio of substrates, enzyme dosage, reaction temperature, and reaction time were evaluated.The results showed that the optimal reaction conditions were as follows:molar ratio of CLA to sunflower seed oil 3 ∶1,enzyme dosage 10%,water content in the system 1%,reaction temperature 55 ℃,reaction time 36 h.Under the optimal condtions,the CLA content and the Sn-2 position CLA content in the finished product were 15.7% and 2.73%,respectively. |
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| Keywords: | CLA CLA immobilized enzyme acidolysis structured lipid containing CLA |
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