| 微生物油脂中花生四烯酸的气相色谱定量检测 |
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| 引用本文： | 董宏祯,汤培平,许建中,许晨.微生物油脂中花生四烯酸的气相色谱定量检测[J].中国油脂,2011,36(9). |
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| 作者姓名： | 董宏祯 汤培平 许建中 许晨 |
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| 作者单位： | 1. 厦门大学化学化工学院,福建厦门,361005
2. 国家海洋局第三海洋研究所,福建厦门,361005 |
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| 基金项目： | 厦门海洋研究开发院共建项目 |
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| 摘 要： | 建立了快速、灵敏测定微生物油脂中花生四烯酸(AA)含量的气相色谱法(GC).采用HP-INNOWAX毛细管柱,氢火焰离子化检测器(FID),优化了分析花生四烯酸酯化产物的色谱条件.结果表明:花生四烯酸甲酯(AA - ME)在0～1.00 mg/mL范围内具有较好线性,相关系数为0.999 6,检测限为0.000 5 mg/mL,相对标准偏差(RSD)为1.49％,回收率在95.86％～105.32％；花生四烯酸乙酯( AA - EE)在0～1.23 mg/mL范围内线性良好,相关系数为0.999 9,检测限为0.003 0 mg/mL,RSD为0.60％,回收率在97.79％～103.61％.
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| 关 键 词： | 气相色谱法 微生物油脂:花生四烯酸:定量检测 |
Quantitative determination of arachidonic acid from microbial oil by gas chromatography |
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| DONG Hongzhen,TANG Peiping,XU Jianzhong,XU Chen.Quantitative determination of arachidonic acid from microbial oil by gas chromatography[J].China Oils and Fats,2011,36(9). |
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| Authors: | DONG Hongzhen TANG Peiping XU Jianzhong XU Chen |
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| Affiliation: | DONG Hongzhen1,TANG Peiping1,XU Jianzhong2,XU Chen2(1.College of Chemistry and Chemical Engineering,Xiamen University,Xiamen 361005,Fujian,China,2.Third Institute of Oceanography,State Oceanic Administration,China) |
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| Abstract: | A gas chromatography(GC) method for the determination of arachidonic acid(AA) in microbial oil was developed.Capillary column HP-INNOWAX with FID was used for detection.The parameters for analyzing esterfied AA were optimized and the method established was proved to be accurate and precise.The linearity range for methyl arachidonate was 0-1.00 mg/mL and the linear correlation coefficient was 0.999 6,the detection limit was 0.000 5 mg/mL,the relative standard deviation(RSD)was 1.49% and the recovery rate was... |
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| Keywords: | gas chromatography microbial oil arachidonic acid quantitative determination |
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