黄精多糖对睡眠干扰诱导小鼠认知功能损伤的作用及机制研究

目的：研究黄精多糖对睡眠干扰(sleep interruption,SI)诱导小鼠认知功能损伤的防治作用，并探讨其作用机制。方法：雄性ICR小鼠72只随机分为空白组、模型组、阳性对照(银杏叶提取物)组，黄精多糖低、中、高(100、200、400 mg/kg)组。预防给药14 d后，除空白组外，其它各组开始为期14 d的睡眠干扰。造模结束后依次进行旷场实验、物体认知实验、避暗实验、Morris水迷宫等行为学检测，并测定血清和海马组织中促炎细胞因子白介素-1β、白介素-6(IL-1β、IL-6)水平；海马组织中神经递质γ-氨基丁酸(GABA)、乙酰胆碱(Ach)水平。结果：旷场实验结果表明各组小鼠运动能力处于同一水平(P>0.05)；与模型组相比，黄精多糖(100、200、400 mg·kg^-1)给药后可以显著改善模型组小鼠在物体认知实验中相对辨别指数(DI)(P<0.05)，延长避暗实验中入暗潜伏期(P<0.05)、减少错误次数(P<0.05)，缩短水迷宫定位航行阶段的寻台潜伏期(P<0.05)；黄精多糖给药组血清和海马中IL-6、IL...

Protective Effects and Mechanism of Polysaccharide from Polygonati Rhizoma on the Cognitive Impairments Induced by Sleep Interruption in Mice

Objective: To study the preventive effect and the related mechanisms of polysaccharide of Polygonati Rhizoma (PSP) on cognitive impairments induced by sleep interruption (SI) in mice. Methods: The 72 ICR mice were randomly divided into the control group, the SI model group, the positive control (ginkgo biloba extract) group and the PSP (100, 200, 400 mg/kg) groups. After 14 days of pretreatment, all groups except the control group received sleep interruption which lasted for 14 days. Thereafter, the behavioral tests were performed by using the open field test, the object recognition experiment, the Morris water maze task and the passive avoidance test. Following the behavioral tests, the biochemical analysis was conducted by measuring the levels of IL-1β and IL-6 in the serum and the hippocampus, the contents of GABA and Ach in the hippocampus. Results: The open field test result showed, there were no significant changes in the locomotor activities of mice among all groups (P>0.05). Compared with the SI model group, SPS (100, 200, 400 mg?kg?1) treatment markedly elevating discrimination index (DI) in object recognition experiment (P<0.05, P<0.01), decreased the error times in entering the dark chamber (P<0.05) and prolonged the latency time in passive avoidance test (P<0.05), as well as decreased the latency period of searching for stations in the navigation stage of water maze positioning (P<0.05). Moreover, PSP administration significantly decreased the levels of IL-1β and IL-6 in the serum and the hippocampus (P<0.05), the content of GABA was significantly decreased (P<0.01), while the level of Ach in the hippocampus was significantly raised (P<0.05). Conclusion: The results reveal that PSP treatment could effectively improve the cognitive impairments induced by SI and its cognition-improving effects might be related to regulate the levels of the proinflammatory cytokines and neurotransmitter in the serum and hippocampus of mice.