参杞酵素发酵工艺优化及调节免疫功能评价

目的：优化参杞酵素的发酵工艺，并研究参杞酵素对小鼠免疫功能的调节作用。方法：以总酸含量为响应值，以发酵时间、发酵温度、菌种比例、接种量为自变量，通过单因素实验和Box-Behnken试验优化发酵工艺。以80 mg/（kg·d）环磷酰胺诱导小鼠构建免疫低下小鼠模型，分别用低（5.0 mL/kg）、中（10.0 mL/kg）、高（15.0 mL/kg）剂量的试验酵素液免疫调节干预，模型组和正常组灌胃等体积的蒸馏水，测定血清中免疫球蛋白G(Immunoglobulin G,IgG)含量和白细胞介素-4(Interleukin-4,IL-4)、白细胞介素-10(Interleukin-10,IL-10)、干扰素-γ(Interferon-γ,IFN-γ)、肿瘤坏死因子-α(Tumor necrosis factor-α,TNF-α)的相对表达量，利用碳廓清法检测小鼠吞噬细胞的吞噬能力及对脏器指数的影响。结果：优化得到发酵工艺为发酵时间72 h，发酵温度37℃，接种量5%，菌种比例5:4.5:0.5，总酸含量为1.23 g/100 g。试验酵素可显著（P<0.05）上调免疫抑制小鼠的血清中...

Optimization of Fermentation Technology and Evaluation of Regulating Immune Function of Ginseng and Wolfberry Fermentation Liquid

Objective: To optimize the fermentation process of ginseng and wolfberry fermentation liquid and study its regulating effect on the immune function of mice. Methods: The fermentation process was optimized by single factor test and Box-Behnken test with total acid content as response value and fermentation time, fermentation temperature, strain proportion and inoculum amount as independent variables. The mice were induced with 80 mg/(kg·d) cyclophosphamide to establish an immunocompromised mouse model. The mice were treated with low (5.0 mL/kg), medium (10.0 mL/kg) and high (15.0 mL/kg) doses of experimental fermentation liquid solution, respectively. The contents of immunoglobulin G (IgG), interleukin-4 (IL-4), interleukin-10 (IL-10) and interferon-γ (interferon-γ) in serum were determined. The relative expression levels of IFN-γ and tumor necrosis factor-α (TNF-α) were measured. The phagocytosis ability of mouse phagocytes and its effect on organ index were detected by carbon clearance method. Results: The fermentation process was optimized as follows: Fermentation time 72 h, fermentation temperature 37 ℃, inoculation amount 5%, strain ratio 5:4.5:0.5, total acid content 1.23 g/100 g. The levels of IFA-γ, TNF-α, IL-4 and IgG in the serum of immunosuppressed mice were significantly up-regulated by the fermentation liquid, and the levels of IL-10 were down-regulated. The mass concentration of IL-10 and IFN-γ in the serum of mice with high dose could be restored to 72.26 and 213.47 pg/mL, respectively. The mass concentration of TNF-α and IgG reached 246.13 pg/mL and 119.89 ng/mL, which were close to or higher than those of solvent control group, and significantly enhanced the phagocytic ability of phagocytic cells. Conclusion: The ginseng and wolfberry fermented liquid had a positive regulatory effect on the immune function of mice.