碳酸酐酶1沉默对肺癌A549细胞增殖、凋亡、侵袭和迁移的影响

目的:探讨沉默碳酸酐酶1（CA1）对人肺癌A549细胞增殖、凋亡、侵袭和迁移的影响。方法:采用脂质体转染法将CA1特异性siRNA（si-CA1组）及阴性对照（si-NC组）转染肺癌A549细胞，同时以转染空脂质体的A549细胞为空白对照组（Blank组）。采用实时荧光定量PCR（qPCR）和蛋白印迹法（Western blot）检测CA1 mRNA和蛋白表达水平，细胞计数试剂盒法（CCK-8）、流式细胞术、Transwell实验分别检测A549细胞增殖、凋亡、侵袭和迁移能力。结果:qPCR和Western blot检测结果显示，转入CA1 siRNA的A549细胞中CA1 mRNA和蛋白的表达水平均明显下调（P<0.05）；CCK-8实验结果显示，沉默CA1后si-CA1组A549细胞在24、48和72 h时光密度（OD）值明显低于si-NC组（P<0.05）；流式细胞术结果发现，si-CA1组A549细胞凋亡率明显高于si-NC组（P<0.05）；Transwell实验结果显示，si-CA1组侵袭和迁移细胞数均明显少于si-NC组（P<0.05）。与Blank组相比，si-NC组以上各指标差异均不显著（P>0.05）。结论:沉默CA1能够抑制肺癌A549细胞增殖、侵袭和迁移，并促进细胞凋亡。

Silencing carbonic anhydrase 1 inhibits the proliferation,invasion and migration of A549 cells in lung cancer

AIM: To investigate the effects of silencing carbonic anhydrase 1 (CA1) on proliferation, apoptosis, invasion and migration of human lung cancer A549 cells. METHODS: CA1-specific siRNA (si-CA1 group) and negative control (si-NC group) were transfected into lung cancer A549 cells by lipofection. The A549 cells transfected with empty liposome were used as blank control group. Real-time quantitative PCR (qPCR) and Western blot (Western blot) were used to detect the expression of CA1 mRNA and protein. Cell counting kit method (CCK-8), flow cytometry and Transwell assay were used to detect proliferation and apoptosis of A549 cells, invasion and migration capabilities. RESULTS:qPCR and Western blot showed that the expression levels of CA1 mRNA and protein in A549 cells transfected with CA1 siRNA were significantly down-regulated (P<0.05). The results of CCK-8 assay showed that silencing of CA1 in Si-CA1 group was observed. The OD values at 24, 48 and 72 h were significantly lower than those in the si-NC group (P<0.05). The results of flow cytometry showed that the apoptosis rate of A549 cells in si-CA1 group was significantly higher than that in si-NC group (P<0.05). Transwell results showed that the number of invading and migrating cells in the si-CA1 group was significantly lower than that in the si-NC group (P<0.05). Compared with the Blank group, the differences of the above indicators in the si-NC group were not significant (P>0.05). CONCLUSION: Silencing CA1 can inhibit the proliferation, invasion and migration of lung cancer A549 cells and promote cell apoptosis.