The incorporation of n-3 polyunsaturated fatty acids into acylglycerols of borage oil via lipase-catalyzed reactions |
| |
Authors: | Yi-Hsu Ju Fang-Cheng Huang Chia-Hui Fang |
| |
Affiliation: | (1) Department of Chemical Engineering, National Taiwan University of Science and Technology, 106 Taipei, Taiwan;(2) Department of Chemical Engineering, Cheng Shiu College of Technology and Commerce, 833 Kaohsiun County, Taiwan |
| |
Abstract: | The purpose of this work was to add n-3 polyunsaturated fatty acids (PUFA) into the acylglycerols of borage oil. The acidolysis reaction between borage oil and n-3 PUFA was carried out with lipase (Lipozyme IM-60) in organic solvent. The effects of temperature, solvent, and water content on the reaction product were investigated. For the acidolysis reaction between acylglycerols (product of the selective hydrolysis of borage oil, catalyzed by immobilized Candida rugosa lipase) and n-3 PUFA, the total content of n-3 and n-6 PUFA in acylglycerols was 72.8% after a reaction time of 18 h. The contents of γ-linolenic acid, eicosapentaenoic acid, and docosahexaenoic acid were 26.5, 19.8, and 18.1%, respectively. By properly controlling the reaction time, acylglycerols with ca. 70–72% PUFA and a ratio of n-3 PUFA to n-6 PUFA from 0–1.09 can be obtained. |
| |
Keywords: | Acidolysis borage oil DHA EPA GLA lipase menhaden oil n-3 PUFA n-6 PUFA |
本文献已被 SpringerLink 等数据库收录! |
|