Guanine oxidation by electron transfer: one- versus two-electron oxidation mechanism |
| |
Authors: | Kupan Adam Saulière Aude Broussy Sylvain Seguy Christel Pratviel Geneviève Meunier Bernard |
| |
Affiliation: | Laboratoire de Chimie de Coordination du CNRS, 205 route de Narbonne, 31077 Toulouse Cedex4, France. |
| |
Abstract: | The degeneracy of the guanine radical cation, which is formed in DNA by oxidation of guanine by electron transfer, was studied by a detailed analysis of the oxidation products of guanine on oligonucleotide duplexes and by labeling experiments. It was shown that imidazolone, the major product of guanine oxidation, is formed through a one-electron oxidation process and incorporates one oxygen atom from O2. The formation of 8-oxo-7,8-dihydroguanine by a two-electron oxidation process was a minor pathway. The two-electron oxidation mechanism was also evidenced by the formation of a tris(hydroxymethyl)aminomethane adduct. |
| |
Keywords: | electron transfer guanine porphyrinoids radical ions riboflavin |
本文献已被 PubMed 等数据库收录! |
|