High-resolution X-ray tomography of the human inner ear: synchrotron radiation-based study of nerve fibre bundles, membranes and ganglion cells |
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Authors: | A. LAREIDA&dagger ,F. BECKMANN&Dagger ,A. SCHROTT-FISCHER§ ,R. GLUECKERT§ ,W. FREYSINGER§ ,& B. MÜ LLER&dagger |
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Affiliation: | Biomaterials Science Center, University of Basel, c/o University Hospital, 4031 Basel, Switzerland;Computer Vision Laboratory, ETH Zürich, Sternwartstrasse 7, 8092 Zürich, Switzerland;Institute for Materials Research, GKSS-Research Center, Max-Planck-Strasse 1, 21502 Geesthacht, Germany;Department of Otorhinolaryngology, Innsbruck Medical University, Anichstrasse 35, 6020 Innsbruck, Austria |
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Abstract: | The combination of osmium tetroxide staining and high-resolution tomographic imaging using monochromatic X rays allows visualizing cellular structures of the human inner ear, that is, the organ of Corti, the stria vascularis and further soft tissues of the membranous labyrinth, in three-dimensional space with isotropic micrometre resolution. This approach permits to follow the course of nerve fibre bundles in a major part of the specimen and reveals the detailed three-dimensional arrangement of individual ganglion cells with distinct nuclei by means of X-ray tomography for the first time. The non-destructive neuron cell counting in a selected volume of 125 μm × 800 μm × 600 μm = 0.06 mm3 gives rise to the estimate that 2000 ganglion cells are present along 1 mm organ of Corti. |
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Keywords: | Cochlea ganglion cell counting osmium stain synchrotron radiation X-ray micro tomography |
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