Quantitative Determination of Deoxynivalenol (DON) Using the Amplified Luminescent Proximity Homogeneous Assay (AlphaLISA) |
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| Authors: | Zhou Bin Wang Ke Jin Jian Chen Yun Huang Biao Zhao Weiguo |
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| Affiliation: | (1) Key Laboratory of Nuclear Medicine, Ministry of Health, Jiangsu Key Laboratory of Molecular Nuclear Medicine, Jiangsu Institute of Nuclear Medicine, Wuxi, Jiangsu, 214063, China;(2) School of Medicine and Pharmaceutics, Jiangnan University, Wuxi, Jiangsu, 214063, China;(3) Shanghai Boyang Biotechnology Company Ltd, Shanghai, 210000, China; |
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| Abstract: | An indirect competitive Amplified Luminescent Proximity Homogeneous Assay (AlphaLISA) was established by using anti-deoxynivalenol
(DON) polyclonal antibody and coating antigen DON-bovine serum albumin (BSA) for detection of DON in cereals. DON-BSA was
coated on Acceptor bead, the kit also contains donor bead sensitizer particles coated with streptomycin. The optional test
conditions and analytical performance of the method were studied. Working concentration ranged from 0.007 to 100 ng/ml and
the sensitivity for detection was 0.007 ng/ml. The intra- and inter-batch coefficient of variation (CV) of the assay were
both below 5%. The recovery rate of artificially contaminated cereals ranged from 81.1% to 110.9% while the mean recovery
of DON from cereals was 94.4%. This study suggests that DON-AlphaLISA method is a good method with high sensitivity and rapidity
for quantitative analyzing DON in cereals. |
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