羟基红花黄色素A对碳离子束辐照致放射性脑损伤的保护作用

探究传统活血化瘀中药红花活性成分羟基红花黄色素A(HSYA)对12C6+重离子束诱发放射性脑损伤的保护作用。昆明种小鼠60只,随机分为正常组、辐照对照组、HSYA低(5 mg/kg)、中(10 mg/kg)、高(20 mg/kg)剂量干预组。给药组于辐照前3 d起每日腹腔注射相应剂量药物,末次给药后1 h,以4.0 Gy12C6+辐照小鼠头部。辐照1个月后,通过Morris水迷宫实验检验小鼠认知功能,以伊文思蓝(EB)作为指示剂测定小鼠血脑屏障通透性,并分别用亚硝酸盐形成法和硫代巴比妥酸(TBA)法测定脑组织中超氧化物歧化酶(SOD)活性及脂质过氧化产物丙二醛(MDA)的含量。结果显示,经12C6+辐照后,单纯照射组小鼠表现出明显的认知功能障碍(P<0.01),小鼠血脑屏障通透性明显升高,脑组织SOD活性明显降低,MDA含量明显增加,羟基红花黄色素A可剂量依赖性地改善小鼠认知功能障碍,保护血脑屏障,上调SOD活性,并降低MDA含量,表明羟基红花黄色素A对12C6+重离子束诱发放射性脑损伤有保护作用。

Protective effects of hydroxysaffor yellow A on brain injury in mice irradiated by 300 MeV/u 12C6+ions

Radiation encephalopathy is the main complication of cranial radiotherapy.It can cause necrosis of brain tissue and cognitive dysfunction,to which no ideal prevention method is available until now.Hydroxysaffor yellow A(HSYA) is the main active ingredient of the traditional Chinese medicine safflower,with protective effects against cerebral ischemic injury.In this work,we investigated the protective effects of HSYA on brain injury in mice irradiated by 300 MeV/u 12C6+ ion beam.The whole head of male Kunming mouse was irradiated to 4.0 Gy after receiving daily intra-peritoneal injection HSYA for 3 d.One month later,the Morris water maze test was used to detect the spatial memory in mice.The Evans blue was used as the tracer to evaluate the permeability of blood-brain barrier.The SOD activity and MDA content in brain tissue were assayed by test kits.The results showed that the12C6+ irradiation significantly impaired the spatial learning and memory in mice,increased the permeability of blood-brain barrier and the MDA content in brain tissue,whereas decreased the SOD activity in brain tissue.The pretreatment with HSYA could improve the spatial memory deficits and inhibit the changes of the blood-brain barrier,the SOD activity and the MDA content in brain tissue in mice.All these demonstrate that HSYA possesses the protective effect against brain injury induced by12C6+particle therapy.