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| 紫菜自由丝状体SCAR标记的获得 | |
| 引用本文： | 柳波,孙彬,金德敏,翁曼丽,许璞,段德麟,王斌,马家海.紫菜自由丝状体SCAR标记的获得[J].高技术通讯,2004,14(12):88-92. |
| 作者姓名： | 柳波 孙彬 金德敏 翁曼丽 许璞 段德麟 王斌 马家海 |
| 作者单位： | 1. 上海水产大学生命科学与技术学院,上海,200090 2. 中国科学院遗传与发育生物学研究所,北京,100101 3. 江苏海洋水产研究所,南通,226007 4. 中国科学院海洋研究所,青岛,266071 |
| 基金项目： | 国家海洋 863计划 (2 0 0 1AA62 10 90 )资助项目 |
| 摘 要： | 应用RAPD技术对27个紫菜自由丝状体材料进行遗传多样性分析，获得7个紫菜材料的7个特异标记。对这7个特异片段进行了回收、克隆，对其中5个片段已完成了序列测定，根据这5个序列的两端顺序合成了5对SCAR—PCR引物，经过优化PCR反应条件成功地将紫菜材料Porphyra tenuipedalis的RAPD扩增片段OPZ-19140和P．yezoensis qd-8的RAPD扩增片段OPJ-18488转换成两个SCAR标记。因为这两个标记的序列已全部测通，故又称为STS标记。这两个SCAR标记(STS标记)可以作为紫菜P．tenuipedalis和P.yezoensis qd-8自由丝状体材料种质鉴定、优良品系选育和产权保护的分子标记。 |
| 关 键 词： | SCAR标记 紫菜 STS标记 丝状体 优良品系 特异片段 遗传多样性分析 RAPD扩增 PCR反应 PCR引物 |
Obtaining of SCAR Markers for Germplasm Identification of Porphyra Lines |
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| Abstract: | Randomly amplified polymorphic DNA (RAPD) technique is applied to studying the polymorphism of 27 Porphyra lines with free-living conchocelises as materials. All together, 13 Porphyra line-specific RAPD markers from 12 Porphyra lines were generated and they were cloned into pGEM-T easy vector respectively. Five of them from 5 important Porphyra lines were successfully sequenced. Five pairs of SCAR-PCR primers were then designed and synthesized according to the 5(-terminal nucleotide sequences of the 5 RAPD markers. Through optimizing the reaction conditions, the specific RAPD markers of P. tenuipedalis and P. yezoensis Yqd-8 were successfully converted into SCAR markers. These will provide a useful way for Porphyra germplasm identification and protection. |
| Keywords: | Germplasm identification SCAR STS RAPD Porphyra Free-living conchocelis lines |
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