Molecular conjugate-mediated gene transfer into isolated human and transplanted rat kidneys

Technically, renal transplantation has been feasible for over four decades. However, immunological injury to the transplanted kidney continues to be the leading cause of graft loss. While current immunosuppressive protocols yield a 1-year graft survival of > 90%, the trade off is increased risks from nephrotoxicity to manifestations of long-term immunosuppression. We have developed techniques which would allow genetic manipulation of the donor kidney while utilizing current procurement and preservation protocols.