提取方法对4种中药雌激素活性的影响

针对4种具有雌激素活性的中药———虎杖、葛根、蛇麻花和女贞子,分别采用水煎和w(C2H5OH)=70%的乙醇水溶液超声法进行提取,用转基因酵母法对其提取产物进行了雌激素活性的测试,对比研究了两种提取方法对4种中药雌激素活性的影响。实验结果显示,虎杖水煎和超声提取样品的EC50分别为3.5×10-3和1×10-3g/L;葛根水煎和超声提取样品的EC50分别为3.5×10-2和1×10-2g/L;女贞子水煎提取样品不显示活性,而超声法提取样品的EC50为2.5×10-3g/L;蛇麻花用这两种方法提取的样品均检测不出雌激素活性。

Influence of Different Methods of Extraction on the Estrogenic Activities of Four Chinese Medicine

Four Chinese medicine—Polygonum cuspidatum,kudzu,Fructus ligustri lucidi and hop were extracted by means of decocting extraction and w(C_2H_5OH)=70% alcohol supersonic extraction separately.The estrogenic activities of their extracts were investigated by a recombinant yeast screening assay(YES).Experimental results demonstrated that EC_(50) of decocting extracts of Polygonum cuspidatum and kudzu were 3.5×10~(-3) g/L and 3.5×10~(-2) g/L respectively,while EC_(50) of alcohol supersonic extracts of them were 1×10~(-3) g/L and 1×10~(-2) g/L.Decocting extract of Fructus ligustri lucidi showed no estrogenic activity,but EC_(50) of its alcohol supersonic extract was 2.5×10~(-3) g/L.The hop extracts of both methods did not show estrogenic activity within the doses tested in this study.