| ^211At通过DTPA酸酐标记IgG |
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| 引用本文： | 刘宁 金建南. ^211At通过DTPA酸酐标记IgG[J]. 核化学与放射化学, 1998, 20(3): 158-163 |
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| 作者姓名： | 刘宁 金建南 |
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| 作者单位： | Institute of Nuclear Science and Technology，Sichuan University，Chengdu 610064 |
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| 摘 要： | 研究了２１１Ａｔ通过二乙撑三胺五乙酸（ＤＴＰＡ）酸酐标记人免疫球蛋白Ｇ（ＩｇＧ）的方法。合成ＤＴＰＡ酸酐后与人ＩｇＧ反应，制得ＤＴＰＡ－ＩｇＧ。Ｎａ２１１Ａｔ溶液与分离纯化后的ＤＴＰＡ－ＩｇＧ在室温下反应３０ｍｉｎ，经ＳｅｐｈａｄｅｘＧ５０柱分离纯化，得２１１Ａｔ－ＤＴＰＡ－ＩｇＧ的０．１ｍｏｌ／ＬＰＢＳ淋洗液。整个标记过程可在１．５ｈ完成，全程标记率在６０％以上。测定了２１１Ａｔ－ＤＴＰＡ－ＩｇＧ在体外的稳定性，并通过比较标记物与Ｎａ２１１Ａｔ注射液在ＮＩＨ小鼠体内的分布和代谢，评价了２１１Ａｔ－ＤＴＰＡ－ＩｇＧ在体内的稳定性。
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| 关 键 词： | ~(211)At 标记方法 DTPA IgG 稳定性 分布 |
LABELING IgG WITH 211 At VIA DTPA ANHYDRIDE |
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| Liu Ning Jin Jiannan Mo Shangwu Chen Hengliu Yu Yanping. LABELING IgG WITH 211 At VIA DTPA ANHYDRIDE[J]. Journal of Nuclear and Radiochemistry, 1998, 20(3): 158-163 |
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| Authors: | Liu Ning Jin Jiannan Mo Shangwu Chen Hengliu Yu Yanping |
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| Abstract: | A method for labeling human IgG with 211 At via DTPA anhydride is described.DTPA IgG is prepared and 211 At is conjugated to human IgG by adding Na 211 At to DTPA IgG and reaction for 30min at room temperature.The astatinated IgG is isolated by a Sephadex G 50 column and identified by size exclusion HPLC.The labeling procedure is executed in 1.5h and at least 60% of the added 211 At are found in the product.The stability of 211 At DTPA IgG in vitro and vivo is evaluated. |
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| Keywords: | At Labeling method DTPA IgG Stability Biodistribution |
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