聚酰胺胺树形分子的叶酸修饰及其表征 |
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引用本文: | 吴江渝,董兴梅,肖夏.聚酰胺胺树形分子的叶酸修饰及其表征[J].武汉工程大学学报,2013,35(1):36-40. |
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作者姓名: | 吴江渝 董兴梅 肖夏 |
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作者单位: | 武汉工程大学材料科学与工程学院,湖北武汉,430074 |
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基金项目: | 国家自然科学基金,武汉市青年科技晨光计划项目 |
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摘 要: | 聚酰胺胺(Polyamidoamine,PAMAM)树形分子是一种广泛用于药物载体和基因载体研究的合成大分子.由于肿瘤细胞表面具有大量表达的叶酸受体,在树形分子末端接枝上叶酸分子可以增强该化合物的肿瘤靶向性.通过发散法合成了0到4代乙二胺为核的聚酰胺胺树形分子并进行叶酸接枝修饰.利用红外光谱及紫外-可见光谱分析,对所得到的树形分子-叶酸化合物进行了结构表征.结果表明:以吡啶作为催化剂叶酸接枝效果较好,通过与叶酸紫外吸收标准曲线对比计算,零代树形分子-叶酸共轭化合物的平均接枝率约为31%,一代至四代树形分子-叶酸共轭化合物的平均接枝率分别为28%、36%、15%和10%.
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关 键 词: | 树形分子 叶酸 肿瘤靶向性 红外光谱 紫外-可见光谱 接枝率 |
Synthesis and characterization of folic acid-modified polyamidoamine dendrimers |
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Authors: | WU Jiang-yu DONG Xing-mei XIAO Xia |
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Affiliation: | (School of Materials Science and Engineering,Wuhan Institute of Technology,Wuhan 430074,China) |
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Abstract: | Polyamidoamine (PAMAM) dendrimers are synthetic molecules which are now widely used as vectors in drugs delivery and gene transfer research. Since folic acid (FA) receptor is over-expressed on the surface of tumor cells, the tumor-targeting effect of dendrimers can be improved though grafting folic acid at the terminal of the dendrimer molecules. Ethylenediamine-core PAMAM dendrimers with generation 0 through 4 were synthesized using divergent strategy, and folic acid molecules were grafted onto the dendrimer surface. The obtained dendrimer-FA compounds were characterized by FT-IR and UV-vis spectra. The results show that pyridine is a good catalyst for the folic acid grafting. The grafting ratio of G0-PAMAM-FA is about 31% and the ratios for the grafted G0-G4 PAMAM dendrimers are 28%, 36%, 15% and 10% respectively. |
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Keywords: | dendrimer folic acid tumor targeting FT-IR spectra UV-vis spectra grafting ratio |
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