诸葛菜种子水提物对急性酒精肝损伤小鼠的保护作用

本文研究了诸葛菜种子水提物对急性酒精肝损伤的保护作用。将雄性ICR小鼠随机分为6组:正常组、模型组、诸葛菜种子水提物低(125 mg/kg),中(250 mg/kg),高(500 mg/kg)剂量组及联苯双酯(150 mg/kg)阳性对照组。连续灌胃给药4 d,末次给药1 h后,通过灌胃56°红星二锅头12 m L/kg建立小鼠急性酒精肝损伤模型,正常组给予等体积蒸馏水。16 h后处死小鼠,检测血清中ALT、AST活性及TG的含量;肝组织中SOD、ADH、GSH-Px及GSH活性;计算肝脏脏器指数;HE染色观察小鼠肝组织的病理变化。结果表明,与模型组相比,诸葛菜种子水提物可显著降低酒精所致急性肝损伤小鼠血清ALT、AST活性及TG含量(p0.05或p0.01),还明显抑制酒精肝损伤小鼠的肝脂肪变性,同时低、中剂量组显著升高肝脏组织匀浆上清中SOD、ADH、GSH-Px和GSH活性(p0.05,p0.01)。因此,诸葛菜种子水提物对小鼠急性酒精性肝损伤具有明显的保护作用。

Protective Effect of Water Extracts of Orychophragmus violaceus Seeds on Alcohol-induced Acute Liver Injury in Mice

The mouse model was used to study the protective effect of water extracts of Orychophragmus violaceus seeds on alcohol-induced acute liver damage. Male ICR (Institute for Cancer Research) mice were randomly divided into six groups: normal group, model group, low-dose water extract group (125 mg/kg), medium-dose water extract group (250 mg/kg), high-dose water extract group (500 mg/kg), and bifendate (150 mg/kg) positive control group. All groups were administered the respective extracts or drug continuously for four days, except for the normal and model groups. Then, alcohol-induced acute liver injury was established in the model and experimental groups by giving each mouse 12 mL/kg 56° Red Star Erguotou at 1 h after the last extract/drug administration. The animals in the normal group were given an equal volume of distilled water instead. The mice were sacrificed after 16 h, and the activities of several key enzymes (the alanine transaminase (ALT), aspartate transaminase (AST), triglyceride (TG) and those in the liver tissue (superoxide dismutase (SOD), alcohol dehydrogenase (ADH), glutathione peroxidase (GSH-Px), and glutathione (GSH) ), were determined. The liver organ index was calculated, and hematoxylin and eosin staining was used to observe the pathological changes in the liver tissue. The seed water extracts could significantly reduce the serum ALT and AST activities and TG level (p<0.05 or p<0.01), and suppress liver steatosis in the mice with alcoholic liver injury, relative to the results in the model mice. Meanwhile, the activities of SOD, ADH, GSH-Px, and GSH in the liver tissue homogenate supernatant were significantly increased in the low- and medium-dose groups (p<0.05, p<0.01). In conclusion, the water extract of Orychophragmus violaceus seeds had a significant protective effect on acute alcoholic liver injury in mice.