| α-淀粉酶的耐酸性改造及在枯草芽孢杆菌中的分泌表达 |
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| 引用本文： | 蔡恒,陈忠军,李金霞,路福平,杜连祥.α-淀粉酶的耐酸性改造及在枯草芽孢杆菌中的分泌表达[J].食品与发酵工业,2005,31(10):33-36. |
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| 作者姓名： | 蔡恒 陈忠军 李金霞 路福平 杜连祥 |
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| 作者单位： | 天津市工业微生物重点实验室,天津科技大学生物工程学院,天津,300222 |
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| 摘 要： | α-淀粉酶基因经改造后,克隆到穿梭质粒pBE2中。在α-淀粉酶基因的上游连接枯草芽孢杆菌sacB基因的启动子-信号肽序列(sacR),构建了含突变α-淀粉酶基因的分泌型诱导表达载体pBSAT,转化蛋白酶三缺陷枯草芽孢杆菌菌株DB403。含有pBSAT的菌株可将突变α-淀粉酶分泌到胞外,表明sacB基因的启动子-信号肽序列(sacR)能很好的将枯草芽孢杆菌中的重组α-淀粉酶引导到胞外,完成分泌表达。分泌的α-淀粉酶具有较高的耐酸性及生物学活性。
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| 关 键 词： | 定点突变 枯草杆菌 α-淀粉酶基因 分泌表达 |
| 收稿时间： | 04 28 2005 12:00AM |
| 修稿时间： | 07 8 2005 12:00AM |
Alteration of Acid-resistance of α-amylase and Secreting Expression in B.subtilis |
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| Cai Heng,Chen Zhongjun,Li Jingxia,Lu Fuping,Du Lianxiang.Alteration of Acid-resistance of α-amylase and Secreting Expression in B.subtilis[J].Food and Fermentation Industries,2005,31(10):33-36. |
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| Authors: | Cai Heng Chen Zhongjun Li Jingxia Lu Fuping Du Lianxiang |
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| Abstract: | The α-amylase gene was mutated and cloned into a E.coli- B.subtilis shuttle vector pBE2.0. Meanwhile the promoter and signal peptide of sacB gene of B.subtilis was linked, resulting in the secreting expression vector pBSAT. The recombinant plasmids were transformed into B.subtilis DB403 and the strain containing pBSAT plasmid can secret α-amylase into the medium. The secreting amylase was of biological activity and acid-resistance. |
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| Keywords: | site-directed mutation B subtilis α-amylase gene secreting expression |
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