赤魟肝脏活性蛋白体外抗氧化作用

采用硫酸铵沉淀、Sephadex G-150 柱层析的方法从赤魟肝脏分离活性蛋白，测定活性蛋白清除超氧阴离子自由基、羟自由基和抑制脂质过氧化作用。结果显示：分离得到的3 个蛋白峰P1、P2 和P3 都具有清除超氧阴离子自由基、羟自由基和抑制脂质过氧化作用，P2 和P3 的抑制作用明显大于P1，P3 的抑制作用最强。实验表明赤魟肝脏活性蛋白具有抗氧化作用。

Antioxidation of Active Proteins from Dasyatis akajei Liver in vitro

The active proteins from Dasyatis akajei liver were isolated by precipitating with ammonium sulfate, chromatographing with Sephadex G-150. Then the activities of scavenging superoxide radical and hydroxyl radical and inhibiting lipid peroxidation were determined. The results showed that the isolated three protein peaks (P1, P2 and P3) could effectively scavenge superoxide radical and hydroxyl radical and inhibit lipid peroxidation. The inhibiting ability of P3 is the highest, while that of P1 is the lowest. In conclusion, the active proteins from Dasyatis akajei liver have antioxidation.