| 阿魏酸修饰的血红蛋白自氧化的研究(加急) |
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| 引用本文： | 王翔,王培. 阿魏酸修饰的血红蛋白自氧化的研究(加急)[J]. 精细化工, 2016, 33(4) |
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| 作者姓名： | 王翔 王培 |
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| 作者单位： | 天津工业大学,天津工业大学 |
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| 基金项目： | 国家自然科学基金资助项目（21202117/B020703） |
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| 摘 要： | 为研究FA-Hb的自氧化，以阿魏酸酯（FA-NBA）与血红蛋白（Hb）为原料，制备了阿魏酸血红蛋白（FA-Hb）。通过紫外光谱扫描、血氧分析仪等检测手段，分析了修饰前后血红蛋白的特征吸收峰的变化情况和氧饱和曲线的差别；研究了有无叠氮钠、黄嘌呤-黄嘌呤氧化酶介导下，血红蛋白和阿魏酸修饰的血红蛋白的自氧化速率的改变。实验结果表明，阿魏酸修饰血红蛋白，保持了血红蛋白的功能结构，仍具备血红蛋白的载氧活性，有效降低了自氧化速率。
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| 关 键 词： | 血红蛋白，阿魏酸，自氧化，高铁血红蛋白，活性氧 |
| 收稿时间： | 2015-12-28 |
| 修稿时间： | 2016-02-17 |
Role of the Ferulic-acid-esterase-ylated Hemoglobin in the Oxidation and Antioxidation |
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| WANG Xiang and WANG Pei. Role of the Ferulic-acid-esterase-ylated Hemoglobin in the Oxidation and Antioxidation[J]. Fine Chemicals, 2016, 33(4) |
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| Authors: | WANG Xiang and WANG Pei |
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| Affiliation: | Tianjin Polytechnic University,Tianjin Polytechnic University |
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| Abstract: | The hemoglobin site-specifically modified with ferulic acid (FA-Hb) has been synthesized in order to analyze the autoxidation of FA-Hb. The effect on the characteristic absorption and the oxygen saturation curve of FA-Hb was investigated by UV spectro-photometry and oxygen analyzer. Furthermore, the autoxidation rate of Hb and FA-Hb was discussed, with or without nitrogen sodium, xanthine- xanthine oxidase mediated in the system. The results showed that the heme structure of FA-Hb is the same as the hemoglobin. It also demonstrated that the oxygen carrying activity of them is same. But, the autoxidation rate of FA-Hb is much lower than Hb in different systems. |
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| Keywords: | Hemoglobin Ferulic acid Antoxidation MetHb ROS |
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