| 产生淀粉糖化酶菌株的诱变选育 |
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| 引用本文： | 潘明,周永进,张强.产生淀粉糖化酶菌株的诱变选育[J].酿酒科技,2006(10):37-39. |
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| 作者姓名： | 潘明 周永进 张强 |
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| 作者单位： | 四川理工学院生物工程系,四川,自贡,643000 |
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| 摘 要： | 以黑曲霉vin-1为研究对象，通过紫外诱变（UV）和硫酸二乙酯（DES）两次诱变，获得产酶较高的菌株vin-1-24-72，酶活达到527u／g，比原始菌株提高154％，RDA％值为40％。菌株经过7次传代，酶活较稳定。
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| 关 键 词： | 微生物 黑曲霉 突变 曲利苯兰 硫酸二乙酯 |
| 文章编号： | 1001-9286(2006)10-0037-03 |
| 收稿时间： | 2006-08-11 |
| 修稿时间： | 2006年8月11日 |
Mutating of Raw Starch-digesting Glucoamylase Strain |
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| PAN Ming,ZHOU Yong-Jin,ZHANG Qiang.Mutating of Raw Starch-digesting Glucoamylase Strain[J].Liquor-making Science & Technology,2006(10):37-39. |
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| Authors: | PAN Ming ZHOU Yong-Jin ZHANG Qiang |
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| Affiliation: | Sichuan College of Science and Technology, Zigong, Sichuan 643000 China |
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| Abstract: | A mutant strain vin-1-24-72 with high yied of raw starch-digesting glucoamylase was isolated from protoplast of Aspergillus niger vin-1 treated by UV and DES. The raw starch-digesting glucoamylase activity of the strain raised to 527 u/g, which was 154 % higher than the enzyme activity ofprotoplast strain. The RDA were 40 % .The enzyme activity of the mutant strain was very steady cultured by seven times. |
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| Keywords: | microbe A spergillus niger mutant trypan blue DES |
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