壳聚糖复合乙氧基喹啉处理对贮藏早红考密斯梨抗氧化性的影响

探明壳聚糖复合乙氧基喹啉处理对梨贮藏期间抗氧化性和品质的影响。以‘早红考密斯’梨为试材,研究了壳聚糖复合乙氧基喹啉处理对梨贮藏期间自由基清除能力、抗氧化酶、抗氧化物质、品质和黑皮病发病率的影响。结果表明,壳聚糖复合乙氧基喹啉处理抑制了果实硬度和可滴定酸含量的下降,使黑皮病发生率降低了69.9%。该处理提高了果实的抗氧化能力,贮藏120d时,处理果的抗超氧阴离子自由基和抑制羟自由基能力分别比对照高8.4%和2.2%。壳聚糖复合乙氧基喹啉处理也诱导了果实中超氧化物歧化酶、抗坏血酸过氧化物酶、谷胱甘肽还原酶和过氧化物酶活性,保持了较高的总酚、花青素、抗坏血酸、还原型谷胱甘肽含量。综上所述,壳聚糖复合乙氧基喹啉处理明显保持了‘早红考密斯’梨的抗氧化能力和采后品质,并显著抑制了果实病害。 

Effect of chitosan combined with ethoxyquin treatment on antioxidant activity in Pyrus communis L. cv. Early Red Comice during storage

The objective of the study was to clarify the effect of chitosan combined with ethoxyquin treatment on antioxidant activity and quality of pear. Free radical scavenging capacity,antioxidant enzymes,antioxidant substance,quality and superficial scald incidence of European pear(Pyrus communis L. cv. Early Red Comice)treated with chitosan combined with ethoxyquin were investigated. Results indicated that decline of firmness and titratable acid content were inhibited by chitosan combined with ethoxyquin treatment. Compared with the control,chitosan combined with ethoxyquin treatment could reduce the development of superficial scald by69.9%. Chitosan combined with ethoxyquin treatment was beneficial to improving antioxidant activity. Anti-superoxide anion and scavenging hydroxyl free radical capacity treated with chitosan combined with ethoxyquin were 8.4% or 2.2% higher than the control at 120 d of storage,respectively. Chitosan combined with ethoxyquin treatment could induce activities of superoxide dismutase, ascorbate peroxidase, glutathione reductase,peroxidase and keep higher content of total phenol,anthocyanin,vitamin C and reduced glutathione than the control. In conclusion,chitosan combined with ethoxyquin treatment was beneficial to maintaining antioxidant activity and quality of ‘Early Red Comice' pear,and inhibited the fruit disease.