响应面法优化姜黄素的提取工艺及其抗氧化活性的研究

采用响应面法优化了姜黄素的提取工艺,并探讨了姜黄素对超氧阴离子自由基（O2-·）、羟基自由基（·OH）、DPPH自由基、ABTS+自由基的体外抗氧化活性。姜黄素的最佳提取工艺条件为乙醇浓度85%,超声时间50min,液料比20∶1（m L/g）。在此条件下,姜黄素得率为1.538%。体外抗氧化实验表明,三种姜黄素标准品具有较强的抗氧化活性且各有差异,姜黄素提取液对超氧阴离子自由基（O2-·）、羟基自由基（·OH）、DPPH自由基、ABTS+自由基也具有较强的清除能力。 

Study on optimization of extraction process for curcuminoids by response surface method and its antioxidant activity

Response surface methodology was used to optimize the extraction process for curcuminoids. The antioxidant activity of curcuminoids was evaluated using superoxide anion(O2-·),hydroxyl(·OH),DPPH and ABTS+radical scavenging. The results indicated that the optimal extraction conditions were ethanol concentration(V/V) 85%,ultrasonic time 50 min,and material-to-liquid ratio 20∶1(m L/g). Under these extraction conditions,maximum yield of curcuminoids of 1.538% was obtained. The antioxidant experiment in vitro demonstated that the antioxidant activity of three curcumin standards were strong and different. Meanwhile the curcuminoids extract also have a better ability to scavenge superoxide anion(O2-·),hydroxyl(·OH),DPPH and ABTS+radical.