免疫磁分离技术在食源性单增李斯特菌检测中应用的研究进展

食源性单增李斯特菌是李斯特菌属中的唯一能引起人类疾病的病原菌,致死率30%~70%,并严重威胁着人类健康。早期快速准确地检测出食品中可能污染的单增李斯特菌对于减少死亡率非常重要,因此亟需建立一些快速、灵敏和高特异性的检测方法。现有单增李斯特菌的检测方法对未经前增菌的食品样本检测灵敏度较低,限制了这些方法直接用于食品样本中单增李斯特菌的快速检测。免疫磁分离是一种可以短时间内高效富集样本中目的菌的技术,与常用的检测方法结合,可以缩短检测周期,提高检测灵敏度。本文综述了免疫磁分离技术在食源性单核细胞增生性李斯特检测中应用的研究进展。 

Research advance on immunomagnetic separation for detection of foodborne Listeria monocytogenes

Listeria monocytogenes was the only human foodborne pathogen of Listeria,which was responsible for listeriosis with high mortality rate of 30% to 70%. Early prevention for L. monocytogenes was of the utmost importance to reduce the disease mortality. Therefore,it was required to achieve the detection methods with rapidity,sensitivity and high specificity. Detection sensitivity of existing detection methods for L. monocytogenes was low in food samples without pre-enrichment,limiting these methods directly used for rapid detection of L. monocytogenes. Immunomagnetic separation was a technology which could efficiently enrich target bacteria in the sample within short time. This technology combined with commonly used detection methods could shorten the detection period and improve the detection sensitivity. Recent advance of the application of immunomagnetic separation technology for the detection of foodborne L. monocytogenes was reviewed in this paper.