苹果幼果中酚类物质抗氧化活性研究

为研究苹果幼果多酚抗氧化活性强弱,实验采用四种体外抗氧化评价体系:亚铁还原能力（ferric reducing ability of plasma,FRAP）,DPPH法,邻苯三酚自氧化体系及邻二氮菲-Fe3+-H2O2法初步研究其抗氧化活性。结果表明,苹果幼果多酚具有较强的总还原能力,幼果多酚浓度为90μg/m L时,其对DPPH·、O-2·及·OH的最大清除率分别可达96.96%、70.61%和82.20%,且不同浓度幼果多酚与相应浓度阳性对照间差异显著（p<0.05）。相关性分析表明,幼果多酚与Fe SO4当量浓度、DPPH·清除率、O-2·清除率及·OH清除率之间均存在明显的量效关系。苹果幼果多酚是一种优良的天然抗氧化剂和自由基清除剂。 

Antioxidative activities in vitro of polyphenols from unripe apple pomace

The method of ferric reducing ability of plasma( FRAP),the scavenging ability of DPPH·,O-2·and ·OH were performed to assess antioxidative activities in vitro of polyphenols from unripe apple pomace.The results indicated that polyphenols from unripe apple pomace had an excellent total reducing power. When the concentration of unripe apple polyphone was 90μg / m L,the maximum scavenging rate on DPPH·,O-2·and ·OH was 96.96%,70.61% and 82.20%,respectively,which had a significant difference with VCand BHT. The significant correlation( p < 0.05) was found among the content of polyphenols,value of FRAP and scavenging rate of DPPH·,O-2·,·OH.The unripe apple polyphenol extracts was a kind of excellent natural antioxidants and free radical scavenger.