莲房多酚提取工艺优化及其抗氧化活性研究

在单因素实验的基础上,通过正交实验优化了热回流提取莲房多酚的工艺,并通过测定其清除ABTS+自由基、DPPH自由基、OH自由基的能力,还原力,亚铁离子螯合活性及对大鼠脑匀浆脂质过氧化的影响,对莲房多酚的抗氧化活性进行综合研究。结果表明,热回流提取莲房多酚的最佳工艺参数为:乙醇浓度50%,料液比1∶25,提取温度80℃,提取时间为2h,最优条件下的得率是5.23%。莲房多酚的体外抗氧化实验表明莲房多酚对DPPH自由基、ABTS+自由基、OH自由基有很强的清除能力,对亚铁离子的螯合活性和还原力均较强,并能显著抑制大鼠脑匀浆脂质过氧化。本研究表明在该优化工艺下提取的莲房多酚具有较强的抗氧化活性。 

Study on the extraction and antioxidant activity of total phenolic compounds from the seedpod of Nelumbo Nucifera Gaertn

The extraction technology of total phenolic compounds from the seedpod of Nelumbo nucifera Gaertn(TPNU) was studied by single-factor experiments and orthogonal test design. Furthermore,the antioxidant activities of TPNU were evaluated,using various antioxidant assays included the scavenging on DPPH,ABTS+,OH free radicals,reducing power determination,ferrous ion-chelating capacity and influence on lipid peroxidation in rats. The results showed that the optimum extraction conditions were determined by orthogonal experiment as follows:ethanol concentration 50%,solid-liquid ratio 1 ∶25,extraction temperature 80℃ and extraction time2 h,and the extraction yield was 5.23% in these conditions. TPNU had strong scavenging activity on DPPH,ABTS+and OH free radicals,also had strong reducing power and ferrous ion-chelating capacity. In addition,TPNU could significantly inhibit the brain lipid peroxidation in rats. These findings showed that TPNU had strong antioxidant activity under the optimal extraction technology.