大麦虫蛋白质酶解产物对小鼠学习记忆能力和抗疲劳能力的影响

为探讨大麦虫蛋白质酶解产物对KM小鼠学习记忆能力和抗疲劳能力的影响,本文采用Morris水迷宫法、爬竿实验和负重游泳实验分别测试灌胃了不同剂量该酶解产物30d后小鼠学习记忆与抗疲劳的情况。结果显示,Morris水迷宫实验中,未灌胃大麦虫蛋白质酶解产物的对照组潜伏期比低、中、高剂量组长17.2%~62.8%,反应次数少19.7%~164.3%,且中剂量组的潜伏期和反应次数与对照组差异均极显著(p<0.01)。爬竿实验和负重游泳实验中,未灌胃该酶解产物的对照组负重游泳成绩比其他三组低1.9%~16.4%;爬竿成绩低6.2%~29.8%,其中,中剂量组的这两项实验成绩均极显著高于低、高剂量组(p<0.01)。表明大麦虫蛋白质酶解产物具有提高小鼠学习记忆能力和抗疲劳能力的功效,且影响程度与其剂量有关,中剂量(1.67g/(kg·d))为较适剂量。 

Effect of protein hydrolysates from Zophobs morio on learning and memory and anti fatigue abilities of mice

To investigate the effect of different doses of Zophobs morio protein hydrolysates on learning,memory and anti fatigue abilities,KM mice were intragastric administrated 30 days' protein hydrolysates,through using Morris water- maze,climbing pole experiment and weight loading swimming test. Results showed that the incubation period of the control group without being administrated whose reaction times was 19.7% ~164.3% less than the low,middle and high dose groups was 17.2% ~62.8% longer than the other groups in the Morris watermaze,while the incubation period and reaction times of the middle dose group differed significantly from the control group( p < 0.01).At the same time,the control group 's score of weight loading swimming test was 1.9% ~16.4% lower than the other three groups and climbing pole experiment test was 6.2% ~29.8% lower in the climbing pole experiment and weight loading swimming test,and the middle dose group got the significant higher results extremely than the low and high dose group( p < 0.01).It suggested that Zophobs morio protein hydrolysates could enhance mice's learning,memory and anti fatigue abilities which was significantly associated with the dosage intragastric administrated,of which moderate dosage( 1.67 g /( kg·d)) was thought to be the most appropriate.