Automated in situ hybridisation using Tecan robotics: a kick-start for Functional Genomics |
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Authors: | Lanchi Vo Young K. Yun Peter Wright Jeff Labadie |
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Affiliation: | Available online 19 February 2004. |
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Abstract: | High throughput parallel organic synthesis is now a common practice in discovery chemistry research. Argonaut's modular reaction cassette technology is aimed at parallel synthesis of focused small molecule libraries. The Trident™ automated platform has been designed to perform high throughput organic synthesis using this modular reaction cassette under truly inert conditions and in a robust, reproducible manner. This enables one to generate high quality libraries of small molecules and to access chemistry not normally amenable to automation.Recently we have introduced a Sample Processing Station (SPS), which enhances the upstream and downstream sample handling capabilities of the Trident™ platform. Enhanced software capabilities also provide the Trident™ synthesizer with powerful reaction development and optimization capabilities. Application of this technology in the synthesis of a small library of 1,2-diarylbenzimidazoles using solution-phase chemistry is presented. A number of product purification and isolation protocols using polymer-bound scavengers are discussed. |
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Keywords: | Author Keywords: IN SITU HYBRIDISATION GENE EXPRESSION HUMAN GENOME PROJECT ROBOTICS FUNCTIONAL GENOMICS |
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