Direct electrochemical reduction of spinach and desulfovibrio desulfuricans Norway ferredoxins

Electrochemical techniques were used to study the reduction-reoxidation of spinach and Desulfovibrio desulfuricans ferredoxins in the presence of poly- -lysine. A peak corresponding to the reduction of native molecules was detected by differential pulse polarography and voltammetry at the basal-plane pyrolytic graphite electrode. The existence of reduction-reoxidation process was evidenced by cyclic voltammetry. The redox potential values of −0.42±0.01 V (spinach ferredoxin), −0.37±0.01 V (D. desulfuricans ferredoxin I), −0.41±0.01 V (D. desulfuricans ferredoxin II) were obtained. No changes in redox potential values were detected in the pH range 5–9.5. The presence of poly- -lysine promotes the electron transfer between the electrode and the ferredoxin molecules. It is suggested that several factors such as electrostatic interactions and the close environment of clusters can influence the electron transfer rates of heterogeneous exchange between the electrode and the ferredoxin molecules.