枸杞多糖结构及其单糖组分的分析研究

枸杞经乙醚脱脂和Sevag法脱蛋白后,用热水提取并用乙醇沉淀多糖,采用紫外光谱、红外光谱、气相色谱和FID检测器对枸杞多糖结构和功效成分单糖进行光谱分析和气相色谱分析。结果表明:枸杞多糖属于蛋白多糖,构杞多糖存在有官能团如—OH,C—O—C,C=O,-NH_2等,其糖苷键存在β-型糖苷键和α-构型的吡喃糖和呋喃糖。多糖为杂多糖,粗多糖得率为2.04%,采用DB-1701毛细管柱对乙酰化后的单糖能进行很好的色谱分离,其单糖组分至少含有8种以上的单糖:鼠李糖、岩藻糖、阿拉伯糖、木糖、甘露糖、半乳糖、葡萄糖等,其中含量较多的是阿拉伯糖、葡萄糖和半乳糖,它们的摩尔比1.956:0.835:0.629,其余的单糖含量比较低。

The Analysis Study on Polysaccharide Structure and Monosaccharide Constituents from the Lycium Barbarum

The polysaccharide was extracted with hot water from Lycium barbarum and precipitated by ethanol after ethyl ether defatting and Sevag method removing the protein.The structure of Lycium barba- rum polysaccharide and monosaccharide constituents was analyzed by using ultraviolet spectrum,infrared spectrum and gas chromatography with a FID detector analyzed.The results showed that Lycium barbarum was proteoglycan,their structure contained functional groups with—OH—C,C—O,C=O,—NH_2 etc,and two types ofα-andβ-glycosidic bonds with pyranose and furanose.Lycium barbarum polysaccharide was het- eroglycan,the yield of crude polysaccharide was 2.04%.The capillary column DB-1701 had good separation effects to acetylized monosaccharide constituents.Lycium barbarum polysaccharide contained over 8 types of monosaccharides:rhamnose,fucose,gumsugar,xylose,seminose,galactose,glucose etc.Gumsugar,glu- cose and galactose were higher constituents than other monosaccharides.Their molar ratio were 1.956:0. 835:0.629.Other monosaccharides were very low.