Experimental models of nasal hypersensitive reaction

An experimental animal models of nasal hypersensitive reaction had been developed in twelve guinea pigs by intranasal applications of 2.4-toluene diisocynate (TDI). Symptoms, morphological appearance of the nasal mucosal scrapings and tissue pathology were studied, and the histamine content in the turbinates evaluated. The results showed a typical picture of nasal hypersensitive reaction. A plenty of eosinophils and mast cells was found on the surface of the nasal mucosa. A marked infiltration of the eosinophils was seen not only in the subepithalial connective tissue but also in the epithelial layer and in the enlarged venous vessels. Significantly higher levels of histamine were detected in the mucosa from TDI treated animals than in control animals (P < 0.01). We consider the experimental models to be ideal.