Effects of processing on the functional properties of canola/rapeseed protein

Canola rapeseed is a major oilseed in Canada, Europe and Japan. Recently, Generally Recognized As Safe (GRAS) status was granted to low erucic acid rapeseed oil for use in the U.S. market. Commercial oil extraction of the seed results in a meal that contains 44% protein and which has been subjected to considerable heat. The meal is presently utilized as livestock feed supplement. A number of processes for the preparation of protein concentrates and isolates from canola/rapeseeds and meal have been proposed, although none have proven commercially viable. In addition to protein concentration, a successful process must reduce the levels of glucosinolates, phenolics, phytates and fiber. These antinutrients present a barrier to the use of canola/rapeseed protein materials in foods. Processes to produce protein concentrates have included water extraction of undesirable compounds from heat denatured, dehulled seed followed by solvent extraction for oil recovery and the isopropanol washing of dehulled, defatted flours. Isolates have been prepared by traditional alkaline extraction, and by acid or water extractions followed by isoelectric, heat or polyelectrolyte precipitation of the protein. Isolates have been chemically and enzymatically modified to improve fooduse properties. In this paper, the effects of various processing methods on the functional properties of solubility, color and flavor of canola protein products are reviewed. Presented at the 78th American Oil Chemists' Society Annual Meeting, May 17–21, 1987, New Orleans, LA.