预处理玻碳电极线性扫描伏安法测定槲皮素的研究

运用循环伏安法、线性扫描伏安法等测试技术研究了槲皮素在预处理玻碳电极上的电化学行为,建立了一种直接测定槲皮素的电化学分析方法。结果表明,与裸玻碳电极相比,预处理玻碳电极能显著提高槲皮素的氧化峰电流。在优化的实验条件下,氧化峰电流与槲皮素浓度在1.0×10-7～2.0×10-5mol/L范围内呈良好的线性关系,最低检测限为6.2×10-8mol/L。该方法简便、快捷、准确、灵敏度高。本法用于芦丁水解产物槲皮素的测定,效果良好。

Determination of quercetin by linear sweep voltammetry with electrochemical pretreated glassy carbon electrode

The electrochemical behavior of quercetin at the electrochemical pretreated glassy carbon electrode(PGCE) was investigated by cyclic voltammetry(CV) and linear sweep voltammetry(LSV).The experimental parameters were optimized,and a direct electroanalytical method for determining quercetin was developed.The oxidation peak current of quercetin was enhanced significantly at the PGCE,in contrast to that obtained at the bare GCE.Under the optimized experimental conditions,the oxidation peak current was proportional to the quercetin concentration in the ranges of 1×10-7~2×10-5 mol/L with a detection limit of 6.2×10-8 mol/L.The pretreated glassy carbon electrode is easy to be prepared with good reproducibility.The proposed methodology was successfully applied to the detection of quercetin in the hydrolysis of rutin with satisfactory results.