| HPCE法手性拆分(R)-和(S)-3-羟基戊二酸乙酯 |
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| 引用本文： | 章申申,董华平,胡六江,徐新. HPCE法手性拆分(R)-和(S)-3-羟基戊二酸乙酯[J]. 化学试剂, 2012, 34(8): 743-745 |
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| 作者姓名： | 章申申 董华平 胡六江 徐新 |
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| 作者单位： | 绍兴文理学院化学化工学院,浙江绍兴,312000 |
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| 基金项目： | 浙江省科技厅科技计划分析测试基金资助项目，绍兴市科技计划项目重点科研资助项目，浙江省实验教学示范中心实验教学改革项目资助项目，绍兴市教育科学规划课题资助项目 |
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| 摘 要： | 以β-环糊精(β-CD)为手性选择剂,采用毛细管区带电泳(CZE)对(R)-和(S)-3-羟基戊二酸乙酯进行手性拆分。通过考察手性选择剂浓度、电泳缓冲液pH、柱温以及分离电压对分离的影响,建立了比较理想的毛细管电泳手性拆分方法。在30 mmol/Lβ-CD、pH 5.0、柱温25℃以及分离电压20 kV分离条件下,(R)-和(S)-3-羟基戊二酸乙酯得到了完全分离。该分离方法具有较好的重复性和稳定性。
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| 关 键 词： | 毛细管电泳 手性拆分 (R)-3-羟基戊二酸乙酯 β-环糊精 |
Chiral resolution of ( R ) - and ( S ) -ethyl 3-hydroxyglutarate by HPCE |
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| ZHANG Shen-shen , DONG Hua-ping , HU Liu-jiang , XU Xin. Chiral resolution of ( R ) - and ( S ) -ethyl 3-hydroxyglutarate by HPCE[J]. Chemical Reagents, 2012, 34(8): 743-745 |
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| Authors: | ZHANG Shen-shen DONG Hua-ping HU Liu-jiang XU Xin |
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| Affiliation: | (College of Chemistry and Chemical Engineering,Shaoxing University,Shaoxing 312000,China) |
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| Abstract: | (R)-And(S)-ethyl 3-hydroxyglutarate were successfully separated into two enantiomers by high performance capillary electrophoresis after β-cyclodextrin(β-CD) was introduced as a chiral selector.The ideally chiral resolution condition was established by investigation of the effects of the concentration of β-CD,pH of buffer,column temperature and separation voltage on the separation efficiency.The optimum conditions were 30 mmol/L of β-CD,pH 5.0,the voltage of 20 kV at 25 ℃.The results showed that this separation method was reproducible,reliable and stable. |
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| Keywords: | capillary electrophoresis chiral resolution (R)-ethyl 3-hydroxyglutarate β-CD |
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