| 手性流动相添加剂法拆分盐酸依替福林对映体 |
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| 引用本文： | 翟明翚,苏立强,王颖,王伟华.手性流动相添加剂法拆分盐酸依替福林对映体[J].化学试剂,2012,34(8):740-742,749. |
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| 作者姓名： | 翟明翚 苏立强 王颖 王伟华 |
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| 作者单位： | 齐齐哈尔大学化学与化学工程学院,黑龙江齐齐哈尔,161006 |
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| 基金项目： | 黑龙江省自然科学基金资助项目 |
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| 摘 要： | 建立了以羧甲基-β-环糊精(CM-β-CD)作为手性流动相添加剂,高效液相色谱拆分标题化合物的方法。系统考察了流动相中添加剂的种类及浓度、有机修饰剂、pH、三乙胺的含量和流速等因素对拆分的影响。色谱分离条件:流动相(V(甲醇)∶V(乙腈)∶V(水)=15∶70∶15),CM-β-CD浓度为0.6 g/L,三乙胺含量为0.14%(V/V),pH为3.92,流速为0.2mL/min,检测波长214 nm,药物对映体得到良好分离。
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| 关 键 词： | 手性流动相添加剂 羧甲基-β-环糊精 高效液相色谱 盐酸依替福林对映体 |
Chiral separation of etilefrine hydrochloride enantiomers using mobile phase additive |
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| ZHAI Ming-hui
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SU Li-qiang
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WANG Ying
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WANG Wei-hua.Chiral separation of etilefrine hydrochloride enantiomers using mobile phase additive[J].Chemical Reagents,2012,34(8):740-742,749. |
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| Authors: | ZHAI Ming-hui SU Li-qiang WANG Ying WANG Wei-hua |
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| Affiliation: | (College of Chemistry and Chemical Engineering,Qiqihar Uiniversity,Qiqihar 161006,China) |
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| Abstract: | Separation of etilefrine hydrochloride enantiomers by HPLC was established using the carboxymethyl-β-cyclodextrin(CM-β-CD in short) as chiral mobile phase additive.The influences of type and concentration of chiral mobile phase additives,the organic modifier,the pH value,the concentration of triethylamine and the flow rate on the enantiomers separation were investigated.The beest separation was achieved when the mobile phase consist of(V(methanol)∶V(acetonitrile)∶V(water)=15∶70∶15),containing 0.6 g·L-1 CM-β-CD and 0.14%(V/V) triethylamine,pH 3.92,at the flow rate of 0.2 mL·min-1.The detection wavelength was set at 214 nm. |
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| Keywords: | chiral mobile phase additive CM-β-CD HPLC etilefrine hydrochloride enantiomer |
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