Enzymatic grafting of gallate ester onto chitosan: evaluation of antioxidant and antibacterial activities |
| |
Authors: | Lidia Itzincab‐Mejía Alberto López‐Luna Miquel Gimeno Keiko Shirai Eduardo Bárzana |
| |
Affiliation: | 1. Depto. Alimentos y Biotecnología, Facultad de Química, Universidad Nacional Autónoma de México, Ciudad Universitaria, , México D.F, 04510 México;2. Departamento de Biotecnologia, Universidad Autonoma Metropolitana, , Vicentina C.P, 09340 México |
| |
Abstract: | The antioxidant octyl gallate (OG) has been successfully grafted onto chitosan by means of horseradish peroxidase biocatalyst. The maximum gallate incorporation onto chitosan determined by 1H NMR spectroscopy was up to 16 molar%. The resulting materials displayed antioxidant capacities with DPPH radical inhibition percentage up to 23% upon the assay conditions. The grafting of the antioxidant on chitosan enhanced its antimicrobial activity. Minimal inhibitory concentration (MIC) for Escherichia coli was 0.5 g L?1. The native medium molecular weight chitosan alone or grafted with the lowest OG incorporation (ca. 11 molar%) attained 100% inhibition of E. coli, whereas lower inhibition was observed for all other materials 50.7–68.9% corresponding a reduction in the counts from 10.6 to 5.23–3.30 Log CFU mL?1. The inhibition of Listeria monocytogenes was significantly higher (59.8–100%) than that with the Gram negative bacterium reaching the MIC at 0.25 g L?1 with a reduction in the counts from 12.6 Log CFU mL?1 to 5.06–0 Log CFU mL?1. |
| |
Keywords: | Antimicrobial agents antioxidant activity polysaccharides |
|
|