Mixtures of 5-hydroxymethylfurfural,levulinic acid,and formic acid have different impact on H2-producing Clostridium strains |
| |
Authors: | Lucas Diniz Giraldeli Bruna Constante Fonseca Valeria Reginatto |
| |
Affiliation: | Departamento de Química, Universidade de São Paulo – Faculdade de Filosofia Ciências e Letras de Ribeirão Preto - FFCLRP, Av. Bandeirantes 3900, Ribeirão Preto SP, Brazil |
| |
Abstract: | Fermentative hydrogen production allows the use of renewable biomasses as feedstocks. However, biomass saccharification results not only in carbohydrates, but also in products that can inhibit fermentation. Although biomass hydrolysates contain mixtures of inhibitors, most studies are performed with a single inhibitor. This study evaluates how 5-hydroxymethylfurfural (HMF, 0.60 g/L), levulinic acid (LA, 2.10 g/L), and/or formic acid (FA, 0.80 g/L) mixtures affect two H2-producing clostridia, Clostridium beijerinckii Br21 and Clostridium acetobutylicum ATCC 824. Fermentation assays with and without (control) the inhibitors helped to calculate the specific H2 production, substrate consumption, and bacterial cell growth rates for Clostridium beijerinckii Br21 or Clostridium acetobutylicum ATCC 824. HMF + AL, HMF + AF, AL + AF, and HMF + AL + AF mixtures inhibited H2 production by C. beijerinckii Br21 by 58.7, 60.0, 46.9, and 83.0%, respectively, and by C. acetobutylicum ATCC 824 by 68.1, 71.4, 58.2, and 89.0%, respectively. Clostridium acetobutylicum ATCC 824 metabolized HMF more efficiently. However, organic acids and their combination with HMF inhibited H2 production by C. beijerinckii Br21 to a lesser extent, which highlighted that this microorganism is robust for H2 production from biomass hydrolysates. |
| |
Keywords: | Inhibitors Fermentation 5-Hydroxymethylfurfural |
本文献已被 ScienceDirect 等数据库收录! |
|